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smooth muscle cells line ha vsmc  (Innoprot Inc)


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    Innoprot Inc smooth muscle cells line ha vsmc
    Smooth Muscle Cells Line Ha Vsmc, supplied by Innoprot Inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/smooth+muscle+cells+line+ha+vsmc/Human+Aortic+Smooth+Muscle+Cells/pmc12785976-147-3-8
    Average 93 stars, based on 4 article reviews
    smooth muscle cells line ha vsmc - by Bioz Stars, 2026-09
    93/100 stars

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    Article Title: ILK Deletion Protects Against Chronic Kidney Disease-Associated Vascular Damage
    Article Snippet: The human aortic-vascular smooth muscle cells line HA-VSMC (Innoprot; Vizcaya, Spain) was maintained in DMEM culture medium supplemented with high glucose (4,5 g l −1 ), L-glutamine (20 mM) (Lonza; Basilea, Switzerland), antibiotics (penicillin, 100 U mL −1 ; streptomycin, 100 mg mL −1 ) (Fisher Scientific, Thermo Fisher Scientific, Waltham, MA, USA), and 10% fetal bovine serum (Sigma-Aldrich, Merck).



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    Figure 2. lncRNA H19 promoted <t>VSMC</t> proliferation and migration (A) Relative expression of lncRNA H19 in human VSMCs infected with lncRNA H19 lentivirus or vector lentivirus. *P<0.05 versus the vector group (n=5 independent experiments). (B) The proliferation of VSMCs infected with lncRNA H19 lentivirus or vector lentivirus was measured by a CCK-8 assay. *P<0.05 versus the vector group (n=5 independent experiments). (C) The migration of human VSMCs was evaluated by a wound healing assay. Magnification: 100×. (D) Quantitative analysis of the migration distance. *P<0.05 versus the vector group (n=3 independent experiments). All the data are presented as the mean±SD (unpaired, two- tailed Student’s t test).
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    Figure 2. lncRNA H19 promoted <t>VSMC</t> proliferation and migration (A) Relative expression of lncRNA H19 in human VSMCs infected with lncRNA H19 lentivirus or vector lentivirus. *P<0.05 versus the vector group (n=5 independent experiments). (B) The proliferation of VSMCs infected with lncRNA H19 lentivirus or vector lentivirus was measured by a CCK-8 assay. *P<0.05 versus the vector group (n=5 independent experiments). (C) The migration of human VSMCs was evaluated by a wound healing assay. Magnification: 100×. (D) Quantitative analysis of the migration distance. *P<0.05 versus the vector group (n=3 independent experiments). All the data are presented as the mean±SD (unpaired, two- tailed Student’s t test).
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    Figure 2. lncRNA H19 promoted <t>VSMC</t> proliferation and migration (A) Relative expression of lncRNA H19 in human VSMCs infected with lncRNA H19 lentivirus or vector lentivirus. *P<0.05 versus the vector group (n=5 independent experiments). (B) The proliferation of VSMCs infected with lncRNA H19 lentivirus or vector lentivirus was measured by a CCK-8 assay. *P<0.05 versus the vector group (n=5 independent experiments). (C) The migration of human VSMCs was evaluated by a wound healing assay. Magnification: 100×. (D) Quantitative analysis of the migration distance. *P<0.05 versus the vector group (n=3 independent experiments). All the data are presented as the mean±SD (unpaired, two- tailed Student’s t test).
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    Figure 2. lncRNA H19 promoted VSMC proliferation and migration (A) Relative expression of lncRNA H19 in human VSMCs infected with lncRNA H19 lentivirus or vector lentivirus. *P<0.05 versus the vector group (n=5 independent experiments). (B) The proliferation of VSMCs infected with lncRNA H19 lentivirus or vector lentivirus was measured by a CCK-8 assay. *P<0.05 versus the vector group (n=5 independent experiments). (C) The migration of human VSMCs was evaluated by a wound healing assay. Magnification: 100×. (D) Quantitative analysis of the migration distance. *P<0.05 versus the vector group (n=3 independent experiments). All the data are presented as the mean±SD (unpaired, two- tailed Student’s t test).

    Journal: Acta biochimica et biophysica Sinica

    Article Title: lncRNA H19 facilitates vascular neointima formation by targeting miR-125a-3p/FLT1 axis.

    doi: 10.3724/abbs.2024087

    Figure Lengend Snippet: Figure 2. lncRNA H19 promoted VSMC proliferation and migration (A) Relative expression of lncRNA H19 in human VSMCs infected with lncRNA H19 lentivirus or vector lentivirus. *P<0.05 versus the vector group (n=5 independent experiments). (B) The proliferation of VSMCs infected with lncRNA H19 lentivirus or vector lentivirus was measured by a CCK-8 assay. *P<0.05 versus the vector group (n=5 independent experiments). (C) The migration of human VSMCs was evaluated by a wound healing assay. Magnification: 100×. (D) Quantitative analysis of the migration distance. *P<0.05 versus the vector group (n=3 independent experiments). All the data are presented as the mean±SD (unpaired, two- tailed Student’s t test).

    Article Snippet: Cell culture The human aortic vascular smooth muscle cell line T/G HA-VSMCs (ATCC CRL-1999) and human embryonic kidney 293T cells (ATCC CRL-3216) were cultured in Dulbecco’s modified Eagle’s medium (DMEM)/Ham’s F12 medium (Gibco, Carlsbad, USA) supplemented with 10% fetal bovine serum (FBS; PAN Biotech, Aidenbach, Germany) and 1% penicillin/streptomycin sulfate in a 5% CO2 incubator at 37°C.

    Techniques: Migration, Expressing, Infection, Plasmid Preparation, CCK-8 Assay, Wound Healing Assay, Two Tailed Test

    Figure 4. miR-125a-3p inhibited VSMC proliferation and migration (A) miR-125a-3p in human VSMCs treated with NC or miR-125a-3p mimics. *P<0.05 versus the NC group (n=5). (B) The proliferation of human VSMCs treated with NC or miR-125a-3p mimics was measured by a CCK8 assay. *P<0.05 versus the NC group. (C) The migration of human VSMCs treated with NC or miR-125a-3p mimics. Magnification: 100×. (D) Quantitative analysis of the migration distance. *P<0.05 versus the NC group (n=3). (E) The proliferation of human VSMCs treated with vector+NC, H19+NC or H19+miR-125a-3p was measured by a CCK-8 assay. *P<0.05 versus the Vector+NC group; &P<0.05 versus the H19+NC group (n=5). (F) The migration of human VSMCs treated with vector+NC, H19+NC or H19+miR-125a-3p. Magnification: 100×. (G) Quantitative analysis of the migration distance. *P<0.05 versus the vector+NC group; &P<0.05 versus the H19+NC group (n=3).

    Journal: Acta biochimica et biophysica Sinica

    Article Title: lncRNA H19 facilitates vascular neointima formation by targeting miR-125a-3p/FLT1 axis.

    doi: 10.3724/abbs.2024087

    Figure Lengend Snippet: Figure 4. miR-125a-3p inhibited VSMC proliferation and migration (A) miR-125a-3p in human VSMCs treated with NC or miR-125a-3p mimics. *P<0.05 versus the NC group (n=5). (B) The proliferation of human VSMCs treated with NC or miR-125a-3p mimics was measured by a CCK8 assay. *P<0.05 versus the NC group. (C) The migration of human VSMCs treated with NC or miR-125a-3p mimics. Magnification: 100×. (D) Quantitative analysis of the migration distance. *P<0.05 versus the NC group (n=3). (E) The proliferation of human VSMCs treated with vector+NC, H19+NC or H19+miR-125a-3p was measured by a CCK-8 assay. *P<0.05 versus the Vector+NC group; &P<0.05 versus the H19+NC group (n=5). (F) The migration of human VSMCs treated with vector+NC, H19+NC or H19+miR-125a-3p. Magnification: 100×. (G) Quantitative analysis of the migration distance. *P<0.05 versus the vector+NC group; &P<0.05 versus the H19+NC group (n=3).

    Article Snippet: Cell culture The human aortic vascular smooth muscle cell line T/G HA-VSMCs (ATCC CRL-1999) and human embryonic kidney 293T cells (ATCC CRL-3216) were cultured in Dulbecco’s modified Eagle’s medium (DMEM)/Ham’s F12 medium (Gibco, Carlsbad, USA) supplemented with 10% fetal bovine serum (FBS; PAN Biotech, Aidenbach, Germany) and 1% penicillin/streptomycin sulfate in a 5% CO2 incubator at 37°C.

    Techniques: Migration, CCK-8 Assay, Plasmid Preparation

    Figure 5. lncRNA H19 promoted VSMC proliferation and migration by suppressing miR-125a-3p to induce FLT1 expression (A) Bioinformatics analysis of the binding sites between miR-125a-3p and FLT1. (B) Relative mRNA expression of FLT1 in arteries collected from mouse sham carotid arteries and injured carotid arteries. *P<0.05 versus the sham group (n=5). (C) Western blot analysis of FLT1 in human VSMCs treated with NC or miR-125a-3p mimics. (D) Quantitative analysis of the FLT1 protein level. *P<0.05 versus the NC group (n=3). (E) Western blot analysis of FLT1 in human VSMCs. (F) Quantitative analysis of the FLT1 protein level in (D). *P<0.05 versus the vector group (n=3). (G) Dual-luciferase reporter assay of human 293T cells treated with NC mimics, miR-125a-3p mimics, and the FLT1-WT or FLT1-Mut luciferase reporters. *P<0.05 versus the FLT1 (WT)+NC group; &P<0.05 versus the FLT1(WT)+miR-125a-3p+vector group (n=3). (H) Western blot analysis of FLT1 in human VSMCs infected with vector or FLT1 lentivirus. (I) Quantitative analysis of the FLT1 protein level. *P<0.05 versus the vector group (n=3). (J) The proliferation of human VSMCs infected with vector or FLT1 lentivirus was measured by a CCK-8 assay. *P<0.05 versus the vector group (n=5). (K) The migration of human VSMCs infected with vector or FLT1 lentivirus. Magnification: 100×. (L) Quantitative analysis of the migration distance. *P<0.05 versus vector (n=3).

    Journal: Acta biochimica et biophysica Sinica

    Article Title: lncRNA H19 facilitates vascular neointima formation by targeting miR-125a-3p/FLT1 axis.

    doi: 10.3724/abbs.2024087

    Figure Lengend Snippet: Figure 5. lncRNA H19 promoted VSMC proliferation and migration by suppressing miR-125a-3p to induce FLT1 expression (A) Bioinformatics analysis of the binding sites between miR-125a-3p and FLT1. (B) Relative mRNA expression of FLT1 in arteries collected from mouse sham carotid arteries and injured carotid arteries. *P<0.05 versus the sham group (n=5). (C) Western blot analysis of FLT1 in human VSMCs treated with NC or miR-125a-3p mimics. (D) Quantitative analysis of the FLT1 protein level. *P<0.05 versus the NC group (n=3). (E) Western blot analysis of FLT1 in human VSMCs. (F) Quantitative analysis of the FLT1 protein level in (D). *P<0.05 versus the vector group (n=3). (G) Dual-luciferase reporter assay of human 293T cells treated with NC mimics, miR-125a-3p mimics, and the FLT1-WT or FLT1-Mut luciferase reporters. *P<0.05 versus the FLT1 (WT)+NC group; &P<0.05 versus the FLT1(WT)+miR-125a-3p+vector group (n=3). (H) Western blot analysis of FLT1 in human VSMCs infected with vector or FLT1 lentivirus. (I) Quantitative analysis of the FLT1 protein level. *P<0.05 versus the vector group (n=3). (J) The proliferation of human VSMCs infected with vector or FLT1 lentivirus was measured by a CCK-8 assay. *P<0.05 versus the vector group (n=5). (K) The migration of human VSMCs infected with vector or FLT1 lentivirus. Magnification: 100×. (L) Quantitative analysis of the migration distance. *P<0.05 versus vector (n=3).

    Article Snippet: Cell culture The human aortic vascular smooth muscle cell line T/G HA-VSMCs (ATCC CRL-1999) and human embryonic kidney 293T cells (ATCC CRL-3216) were cultured in Dulbecco’s modified Eagle’s medium (DMEM)/Ham’s F12 medium (Gibco, Carlsbad, USA) supplemented with 10% fetal bovine serum (FBS; PAN Biotech, Aidenbach, Germany) and 1% penicillin/streptomycin sulfate in a 5% CO2 incubator at 37°C.

    Techniques: Migration, Expressing, Binding Assay, Western Blot, Plasmid Preparation, Luciferase, Reporter Assay, Infection, CCK-8 Assay

    Figure 7. Schematic diagram summarizing the role of the lncRNA H19/miR-125a-3p/FLT1 axis in neointima formation lncRNA H19 overexpression significantly promoted neointima formation in vivo and VSMC proliferation and migration in vitro. Mechanistically, lncRNA H19 acted as a ceRNA of miR-125a-3p and facilitated the expression of the miR-125a-3p target FLT1 in VSMCs.

    Journal: Acta biochimica et biophysica Sinica

    Article Title: lncRNA H19 facilitates vascular neointima formation by targeting miR-125a-3p/FLT1 axis.

    doi: 10.3724/abbs.2024087

    Figure Lengend Snippet: Figure 7. Schematic diagram summarizing the role of the lncRNA H19/miR-125a-3p/FLT1 axis in neointima formation lncRNA H19 overexpression significantly promoted neointima formation in vivo and VSMC proliferation and migration in vitro. Mechanistically, lncRNA H19 acted as a ceRNA of miR-125a-3p and facilitated the expression of the miR-125a-3p target FLT1 in VSMCs.

    Article Snippet: Cell culture The human aortic vascular smooth muscle cell line T/G HA-VSMCs (ATCC CRL-1999) and human embryonic kidney 293T cells (ATCC CRL-3216) were cultured in Dulbecco’s modified Eagle’s medium (DMEM)/Ham’s F12 medium (Gibco, Carlsbad, USA) supplemented with 10% fetal bovine serum (FBS; PAN Biotech, Aidenbach, Germany) and 1% penicillin/streptomycin sulfate in a 5% CO2 incubator at 37°C.

    Techniques: Over Expression, In Vivo, Migration, In Vitro, Expressing